Two-Strain, Cell-Selective Protein Labeling in Mixed Bacterial Cultures
نویسندگان
چکیده
منابع مشابه
Two-strain, cell-selective protein labeling in mixed bacterial cultures.
Cell-selective metabolic labeling of proteins with noncanonical amino acids enables the study of proteomic changes in specified subpopulations of complex multicellular systems. For example, azidonorleucine (Anl) and 2-aminooctynoic acid, both of which are activated by an engineered methionyl-tRNA synthetase (designated NLL-MetRS), are excluded from proteins made in wild-type cells but incorpora...
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ژورنال
عنوان ژورنال: Journal of the American Chemical Society
سال: 2012
ISSN: 0002-7863,1520-5126
DOI: 10.1021/ja3004667